SOP for Calibration of Refractometer?

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Calibration of Refractometer:

1.Objective:

To lay down a procedure for calibration of Refractometer.

2. Scope:

This procedure is applicable to Quality control laboratory for make Anton Paar and Rudolph.

3. Responsibilities:

RoleResponsibilityDepartment
Analyst/Team LeaderCalibration of RefractometerQuality Control
Reviewer/ Reviewer Team LeaderReview of reportsAQA/Lab QA
Head of DepartmentEnsuring compliance of this SOPQuality Control
Calibration of Refractometer

4. Procedure:

  1. Wipe the instrument with lint free cloth till visually clean.
  2. Ensure the power supply and the interface cable connecting the measuring unit are properly connected.
  3. Ensure that the instrument is clean before measuring the sample.
  4. Never overfill the sample well.
  5. Sample on prism shall be free from air bubbles.
  6. After measurement, sample must be removed and wipe off immediately with tissue paper.
  7. After wiping the sample from sample well, wipe again with water incase of water base sample.
  8. For Non water base samples wipe with organic chemicals like Acetone, IPA or Ethanol.

Procedure for Calibration of Refractometer:

Following calibration parameters mentioned for Anton Paar make instrument:

Calibration with reference standard:

Calibrate the instrument using any one of the reference standard at 20°C ± 0.5 °C.

1. Chlorobenzene 2. Tetrachloroethylene

In case of non availability of standards, alternate certified reference standard shall be used with acceptance criteria of ± 0.0001 to certified value.

Acceptance Criteria:

Refractive index of reference standard at 20°C shall be ± 0.0001 to certified value.

Calibration with Purified Water:

Calibrate the instrument using purified water at 20°C ± 0.5 °C.

Acceptance Criteria:

Refractive index of purified water at 20°C shall be 1.33290 to 1.33310.

Temperature variation at 20°C:

Verify the refractive index of water at 20°C ± 0.5 °C

Acceptance Criteria:

Refractive index of purified water at 20°C shall be 1.33290 to 1.33310.

Temperature variation at 25°C:

Verify the refractive index of water at 25°C ± 0.5 °C

Acceptance Criteria:

Refractive index of purified water at 25°C shall be 1.33240 to 1.33260.

Following calibration parameters mentioned for Rodolph Instrument:

Calibration with Reference standard:

Calibrate the instrument using the laser liquid 20°C ± 0.5 °C

Acceptance Criteria:

Refractive index of laser liquid standard at 20°C shall be ± 0.0001 nD to the certified value.

Calibration with Purified Water:

Calibrate the instrument using purified water at 20°C ± 0.5 °C.

Acceptance Criteria:

Refractive index of purified water at 20°C shall be 1.33290 to 1.33310.

Temperature variation at 20°C:

Verify the refractive index of water at 20°C ± 0.5 °C

Acceptance Criteria:

Refractive index of purified water at 20°C shall be 1.33290 to 1.33310.

Temperature variation at 25°C:

Verify the refractive index of water at 25°C ± 0.5 °C

Acceptance Criteria:

Refractive index of purified water at 25°C shall be 1.33240 to 1.33260.

Frequency of Calibration:

Once in six months or after maintenance major job as per service engineer recommendation.

calibration-of-refractometer

Calibration of Refractometer

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