Calibration of Osmometer in Pharma

Calibration Of Osmometer:

3 point calibration of Osmometer:

  1. Turn on the main power supply and then switch on the instrument by using “on/off” switch.
  2. Go to measure on display and click on calibrate.
  3. Click on “Prepare” button to get instrument ready.
  4. Once instrument is ready perform the calibration by using zero point.
  5. Pipetted a 50 μl of Milli Q/HPLC grade water into a fresh measurement vessel.
  6. Measurement vessel cover should be placed front side of the instrument.
  7. Move the elevator down side. Then Zero point calibration will start automatically.
  8. Move the elevator up, remove the measurement vessel from the elevator.
  9. Clean the temperature sensor with Milli Q water using soft, dry, lint free paper towel.
  10. Again run the fresh samples of Milli Q/ HPLC grade water as per above procedure.
  11. After completed of two runs of Milli Q water display will show zero point calibration pass.
  12. Click on the 1st standard and select the first standard solution of 300mOsmol.
  13. Clean the sensor with Milli Q water/HPLC grade water using soft, dry, lint free paper towel.
  14. Pipetted 50 μl of 300mOsmol/kg standard into a fresh measurement vessel, mount it and pull down.
  15. Measurement vessel cover should be placed front side of the instrument.
  16. Move the elevator down side. Then 1st standard calibration will start automatically.
  17. Move the elevator up, then remove the measurement vessel from the elevator.
  18. Clean the temperature sensor with Milli Q water using soft, dry, lint free paper towel.
  19. Again run the fresh samples of 300mOsmol/kg standard as per above procedure.
  20. After completion of two runs with 300 mOsmol/kg, display will show 1st standard was successful.
  21. Click for 2nd standard.
  22. Clean the sensor with Milli Q water/HPLC grade water using soft, dry, lint free paper towel.
  23. Pipetted 50 μl of 850mOsmol/kg standard into a fresh measurement vessel, mount it and pull down.
  24. Measurement vessel cover should be placed front side of the instrument.
  25. Move the elevator down side. Then 1st standard calibration will start automatically.
  26. Move the elevator up, then remove the measurement vessel from the elevator.
  27. Clean the temperature sensor with Milli Q water using soft, dry, lint free paper towel.
  28. After completion of two runs with 850 mOsmol/kg, display will show 2nd standard was successful.
  29. Then click on “Finish” button to finish the calibration.

Acceptance Criteria of 3 point Calibration:

  1. For Zero Point: The value should be within -2 to +2.
  2. For 300 Standard: The value should be within 298 to 302
  3. For 850 Standard: The value should be within 846 to 854

Daily Verification:

After 3 point calibration of osmometer, daily verification shall be perform. The verification shall be perform by Using the calibration Of 290 mOsmol/Kg Standard.

Acceptance criteria: The value 290 mOsmol/kg standard should be within 288 to 292 mOsmol/kg.

Osmometer Calibration frequency:

3 point Calibration of osmometer shall be performed once in six months and as recommended by the service engineer after maintenance activity.

calibration of osmometer

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