Calibration Of Preparative HPLC Pump

Calibration of preparative HPLC pump:

Objective:

To lay down the procedure for the preparation and calibration of preparative HPLC pump.

Scope:

This SOP applies to the Quality control department.

Responsibilities:

Role	Responsibility	Department
Analyst	Calibration of Refractometer	Quality Control (QC)
Reviewer/ Reviewer Team Leader	Review of reports	Quality Assurance (QA)
Head of Department	Ensuring compliance of this SOP	Quality Control (QC)

Calibration of preparative HPLC pump

Procedure:

To maintain the flow accuracy degas the solvent before use.
Setting the flow rate
Press the flow key and enter the numerical value for the flow rate in ml/min.
Press enter, and then run. Subsequent changes in the flow rate require only entering the numerical value and pressing enter. changes in flow may also be made by using the up/down scroll key.
Priming the Pump
Open the purge valve 1/2 turn.
Press purge function.
The pump will draw solvent through the inlet lines and expel air out the purge valve to waste. When no more air is present in the intake lines and cannot be seen coming out of the purge valve waste port, then the pump is primed.

Make sure that all flow lines are connected to the pump and put the pump inlet tube in the water.
Make a readily available volumetric flask before starting the procedure.
After connecting all the flow lines of a pump and choose four different flow rates 1ml/min, 2ml/min, 4ml/min and 8ml/min within the allowed range of the pump.
Start the pump and make water flow through the flow lines.
Measure the out water for 1 min in the volumetric flask.
record the details in the given format.

Acceptance Criteria:

The difference between actual measured value and set value should be ± 2%.

Frequency:

Once in a 6 months.

Calibration of Preparative HPLC Column

Also Read:

Refer YT Channel: Pharmabeejpro