UV-Visible Spectrophotometer Interview Questions:
UV spectroscopy or UV-visible spectroscopy is an absorption spectroscopy or reflectance spectroscopy or transmittance spectroscopy. It is a technology used in many areas, including drug identification, and bacterial culturing, to check purity, in chemicals as well as in the pharmaceutical industry.
Let’s start with UV-visible spectrophotometer interview questions and answers.
Q1: What is a UV-visible spectrophotometer?
A: UV-visible spectrophotometer is an analytical instrument that measures the ultraviolet and visible light that is absorbed by the sample.
Q2. What is the scan range of the UV region?
A: The scan range for the UV region is 200nm-400nm.
Q3 What is the scan range of the visible region?
A: The scan range of visible range is 400nm-800nm.
Q4: What is the Principle of Beer law?
A: Absorption is directly proportional to the concentration of the medium. i.e. more the concentration more absorption.
Q5: What is the Principle of Lambert’s Law?
A: Absorption is directly proportional to the path length of the cell. i.e. the larger the path length size more the absorption.
Q6: What is the principle of UV-visible spectroscopy?
A: When a beam of monochromatic light passes through a homogeneous absorbing medium, the rate of decrease in intensity of light is directly proportional to the concentration and path length of the cell.
Q7: How many types of cuvettes are used in UV-visible spectrophotometers?
A: There are 4 most common types of cuvettes used in UV visible spectroscopy.
Q8: What are the 4 types of cuvettes?
A: 1. Optical Glass or Pyrex Glass 2. UV quartz 3. IR quartz 4. Sapphire
Q9: What type of cuvettes used in UV region?
A: Quartz cuvette is used in the UV region.
Q10: What type of cuvette is used in visible region?
A: Glass cuvette is used in the visible region.
Q11: What type of analysis can be performed on UV UV-visible spectrophotometer?
A: Both, qualitative and quantitative types of analysis can be performed on a UV-visible spectrophotometer.
Q12: What is the light source for the UV region?
A: Deuterium Lamp is used as a light source in the UV region.
Q13: What is the light source for the visible region?
A: A Halogen Lamp is used as a light source in the UV region.
Q14: Which type of lamp is used in a UV-visible spectrophotometer?
A: Xenon lamp is used in UV-visible spectrophotometer.
Q15: What are the parameters used for UV calibration?
A: Control of wavelength, Control of absorption, Limit of stray light, Resolution, Baseline flatness.
Q16: Why UV-Visible spectroscopy is used?
A: A UV visible spectroscopy is used to determine the concentration of the molecules in the absorbing solution.
Q17: What is the difference between a single-beam and a double-beam spectrophotometer?
A: A single-beam spectrophotometer has only one beam of light while a double-beam has two beams of light.
Q18: What is the Instrumentation of UV-visible spectroscopy?
A: Light Source, Monochromator, Sample holder, Detector and recorder.
Q19: Why quartz cuvette is used in the UV scanning range?
A: Because the quartz cuvette is not absorbing the light in UV region.
Q20: What is the general chapter number of UV visible spectroscopy as per USP?
A: USP general chapter number 857.
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