UPLC Advantages Over HPLC:
Even though the working principle of UPLC and HPLC are the same but UPLC is a more advanced separation technique as compared to HPLC.
Let’s understand the what are the UPLC advantages over HPLC?
- Speed
- Resolution
- Specificity
- Time and Cost Effective
Speed:
- Reduction in solvent consumption
- Increased sample throughout
Resolution:
- The enhanced sensitivity allows lower levels of analysis
- With smaller particle sizes and good peak separation, you can analyze samples with multiple analytes in difficult matrices
Specificity:
Detailed and sharp peaks – better peak separation
Time and Cost Effective:
- Operating at a higher pressure
- Not just reducing solvent consumption, but reducing storage and disposal as well
- A shorter run time, improved sensitivity, and selectivity of the analytes allow for a higher sample throughout
Also Read:
- HPLC Principle and Application
- Application of Karl Fischer Titration
- Principle & Application of IR Spectroscopy
- What is Validation in pharma
- Definition of ALCOA Plus
Refer YT Channel: Pharmabeej