What are the 5 components of HPLC in pharma?

What are the components of HPLC?

As HPLC chromatography is a widely used technique for the separation of components from the mixtures but to separate those components from the mixture of HPLC components has a major role.

There are major 5 components of HPLC as follows:

  1. Solvent reservoir
  2. Pump system
  3. Injection port
  4. HPLC Column
  5. HPLC Detector

1. Solvent Reservoir:

The solvent reservoir holds a solvent that is used to carry the sample solution through a column called as mobile phase.

2. Pump System:

A pump system is used to push the mobile phase at a given flow rate.

3. Injection Port:

The injection port is used to introduce a sample solution into the column.

4. HPLC Column:

The column is a key component in the HPLC. The column is a tube that contains a stationary phase, where the reaction takes place.

4. HPLC Detector:

A detector is a component where eluted components can be detected in the form of chromatograms.

Components of HPLC

Also Read:

HPLC Interview Question and Answers

HPLC Calibration Parameters in pharma

Gas Chromatography Interview questions and answers

Karl Fischer Titration Interview Question and Answer

Dissolution Interview Question and Answer

Interview Question and answer on Polarimeter

For Interview Preparation Refer: Interview

For interview preparation refer YT channel: Pharmabeej