What are the components of HPLC?
As HPLC chromatography is a widely used technique for the separation of components from the mixtures but to separate those components from the mixture of HPLC components has a major role.
There are major 5 components of HPLC as follows:
- Solvent reservoir
- Pump system
- Injection port
- HPLC Column
- HPLC Detector
1. Solvent Reservoir:
The solvent reservoir holds a solvent that is used to carry the sample solution through a column called as mobile phase.
2. Pump System:
A pump system is used to push the mobile phase at a given flow rate.
3. Injection Port:
The injection port is used to introduce a sample solution into the column.
4. HPLC Column:
The column is a key component in the HPLC. The column is a tube that contains a stationary phase, where the reaction takes place.
4. HPLC Detector:
A detector is a component where eluted components can be detected in the form of chromatograms.
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